Gambaran Makroskopis dan Pewarnaan Histologi HE Pada Tikus Wistar Model Alopesia Like yang Diinduksi Fluconazole

Abstract

Fluconazole-induced alopecia is an increasingly common side effect reported in long-term therapy, both in humans and experimental animals. The aim of this study was to determine the macroscopic appearance and histological staining results of hematoxylin-eosin (HE) in fluconazole-induced Wistar rats. The method used was an in vivo experimental study with a Post Test Only Control Group Design. Male Wistar rats were divided into a fluconazole-induced treatment group and a healthy control group. Skin tissue analysis was performed on the 14th day to observe the macroscopic appearance and HE staining results. The results showed that macroscopically, the treatment group showed a slowdown in hair growth compared to the healthy control group. HE staining showed that administration of 35 mg/kgBW of fluconazole for 14 days caused a reduction in hair follicles in the anagen phase. In conclusion, fluconazole administration caused a slowdown in hair growth and disruption of anagen phase follicles in Wistar rats.